ISSN (Online) : 2456 - 0774

Email : ijasret@gmail.com

ISSN (Online) 2456 - 0774

A STUDY ON SCREENING ,OPTIMIZATION, PRODUCTION AND APPLICATION OF DEXTRAN  FROM WEISSELLA CIBARIA

Abstract

Dextran a high molecular weight exopolysaccharide with significant industrial and biomedical applications is predominantlyproduced by latic acid bacteria. This study focuses on the screening, optimization, production, and application of dextranfromWeissellacibaria a promising dextran production microorganism. Intially various Weissella cibaria were screened for dextranproductionusingsucrose based media with W. cibaria demonstrating superior yield and polymer quality. Optimization of culture conditionsuchaspH,temperature, incubation, time and sucrose concentration was carried out using Response Surface Methodology (RSM) tomaximizedextran yield. The optimized condition resulted in a significant enhancement of dextran production, confirmed throughgravimetricandspectrophotometric analyses. Structural characterization via FTIR and NMR confirmed the polymeric nature and functional groupsofthedextran produced. Finally the application potential of the produced dextran was evaluated in food and pharamaceutical contexts,particularly as a prebiotic and a stabilizing/thickening agent. The study demonstrates the feasibility of using W. cibaria as asustainablemicrobial source for high quality dextran with potential for scalable industrial production

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