Mukia maderaspatana, a medicinal plant with significant ethno pharmacological relevance, was investigated for its antiinflammatory, antioxidative, and antihyperlipidemic properties. Soxhlet-assisted hot extraction and cold maceration were employed toobtain bioactive-rich fractions. Phytochemical characterization was performed using advanced analytical techniques, including GasChromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) for compound identification,Fourier Transform Infrared Spectroscopy (FTIR) for functional group analysis, Ultraviolet-Visible Spectroscopy (UV-Vis) for electronictransitions, and Mass Spectrometry for molecular weight determination. The most promising bioactive compounds were identified basedon spectral interpretation and their potential pharmacological relevance. The extracts were subjected to in vitro evaluations to determinetheir efficacy in modulating inflammatory mediators, and regulating lipid metabolism. The anti-inflammatory potential was assessedthrough inhibition of key pro-inflammatory markers, while antioxidative activity was determined. The antihyperlipidemic effect wasevaluated based on lipid profile modulation. The results demonstrated significant therapeutic potential, supporting the plant’s role in diseaseintervention. This study highlights the pharmacological significance of Mukia maderaspatana as a natural therapeutic agent, emphasizingits potential for further drug development. The novelty of this research lies in the identification of its bioactive constituents and their targetedpharmacological effects, reinforcing its role in herbal medicine as a viable candidate for managing inflammatory, oxidative, and lipidassociated disorders.Keywords: Mukia maderaspatana, anti-inflammatory, antioxidative, antihyperlipidemic, phytochemicals, in vitro study, herbal medicine.