The Aspartic acid-Cu(II) complex is a coordination compound formed by the binding of copper(II) ions with aspartic acid, anessential amino acid, exhibiting potential biological and pharmacological applications. This study investigates the complexation behaviorbetween aspartic acid and Cu(II) ions using potentiometric titration methods at varying temperatures (303K, 308K, and 313K) undercontrolled ionic strength conditions. The stability constants of the Cu(II)-aspartic acid complex were determined using the modified IrvingRossotti technique. Thermodynamic parameters including Gibbs free energy (ΔG), enthalpy change (ΔH), and entropy change (ΔS) werecalculated to understand the spontaneity and nature of the complexation process. The proton-ligand stability constants (pKa) and metalligand stability constants (log K) were evaluated at different temperatures. Additionally, the antimicrobial activity of the synthesizedcomplex was assessed against selected bacterial strains using standard protocols to explore its potential therapeutic applications. Thecomplex formation was confirmed through various physicochemical techniques, and the results indicate the formation of stable coordinationcompounds under the experimental conditions.Keywords: Aspartic acid-Cu(II) complex, Complexation, Potentiometric method, Stability constant, Antimicrobial effect etc.