Soil stabilization is an essential aspect of geotechnical engineering, particularly for black cotton soil, which exhibits significantvolumetric changes. This study investigates the effectiveness of Bionzyme, an eco-friendly soil stabilizer, in improving soil properties.Various laboratory tests, including Unconfined Compressive Strength (UCS), California Bearing Ratio (CBR), and Atterberg Limits, wereconducted to evaluate the stabilization effects. The findings suggest that Bionzyme enhances soil strength, reduces swelling, and improvescompaction characteristics, making it a sustainable alternative for soil stabilization.